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After washes with PBS for 3 times, cells were incubated with 1 M DAF-FM DA probe for 20 ~ 30 min

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Consistently, hES-MSCs cultured at 5 10 3 cells/cm 2 (hereafter referred to as high-density) showed enhanced stem cell functions compared to those of cells cultured at 2 10 3 cells/cm 2 (hereafter referred to low-density) from all aspects, including stem cell marker expression, migration, and CFU (Fig

2019), or to support regeneration of GSH oxidized by other compounds such as superoxide (Polle 2001), as well as some of the thiol peroxidases discussed further below